FCC Errata Table - All

In footnote 12: Change http://www.cast-science.org/publications/index.cfm/probiotics_their_pot….
to:
http://www.cast-science.org.

Line 1 of Primer set 1: Change
The PCR sample preparation prepared with Primer set 1 gives an amplification product of 351 base pairs with an SNP identified as (underlined in the following 15 base pair sequence): CTTCAGATTTTAGGC. The SNP location is 44 base pairs from the 5′ end of the forward primer described in Primer set 1. The sequence of the amplicon should be determined by validated, standard sequencing technologies.
to:
The PCR sample preparation prepared with Primer set 1 gives an amplification product of 351 base pairs with an SNP identified as (underlined in the following 15 base pair sequence): CTTCAGATTTTAGGC. The SNP location is 44 base pairs from the 5′ end of the forward primer described in Primer set 1. The sequence of the amplicon should be determined by validated, standard sequencing technologies.
AND
Line 1 of Primer set 2: Change
The PCR sample preparation prepared with Primer set 2 gives an amplification product of 531 base pairs with an SNP identified as (underlined in the following 15 base pair sequence): GCCCGCTCAAACGAA. The SNP location is 279 base pairs from the 5′ end of the forward primer described in Primer set 2. The sequence of the amplicon should be determined by validated, standard sequencing technologies.
to:
The PCR sample preparation prepared with Primer set 2 gives an amplification product of 531 base pairs with an SNP identified as (underlined in the following 15 base pair sequence): GCCCGCTCAAACGAA. The SNP location is 279 base pairs from the 5′ end of the forward primer described in Primer set 2. The sequence of the amplicon should be determined by validated, standard sequencing technologies.

Line 1 of Primer set 1: Change
The PCR sample preparation prepared with Primer set 1 gives an amplification product of 365 base pairs with two SNPs. The first SNP is identified as (underlined in the following 15 base pair sequence): GAGTAAATTCCATCA and the location is 49 base pairs from the 5′ end of the forward primer described in Primer set 1. The second SNP is identified as (underlined in the following 15 base pair sequence): TTCAATTGAAGTTTG and the location is 241 base pairs from the 5′ end of the forward primer described in Primer set 1. The sequence of the amplicon should be determined by validated, standard sequencing technologies.
to:
The PCR sample preparation prepared with Primer set 1 gives an amplification product of 365 base pairs with two SNPs. The first SNP is identified as (underlined in the following 15 base pair sequence): GAGTAAATTCCATCA and the location is 49 base pairs from the 5′ end of the forward primer described in Primer set 1. The second SNP is identified as (underlined in the following 15 base pair sequence): TTCAATTGAAGTTTG and the location is 241 base pairs from the 5′ end of the forward primer described in Primer set 1. The sequence of the amplicon should be determined by validated, standard sequencing technologies.
AND
Line 1 of Primer set 2: The PCR sample preparation prepared with Primer set 1 gives an amplification product of 433 base pairs with two SNPs. The first SNP is identified as (underlined in the following 15 base pair sequence): AAGCATTCAATACAC and the location is 72 base pairs from the 5′ end of the forward primer described in Primer set 2. The second SNP is identified as (underlined in the following 15 base pair sequence): ATATGGTTCTTACCT and the location is 215 base pairs from the 5′ end of the forward primer described in Primer set 2. The sequence of the amplicon should be determined by validated, standard sequencing technologies.
to:
The PCR sample preparation prepared with Primer set 1 gives an amplification product of 433 base pairs with two SNPs. The first SNP is identified as (underlined in the following 15 base pair sequence): AAGCATTCAATACAC and the location is 72 base pairs from the 5′ end of the forward primer described in Primer set 2. The second SNP is identified as (underlined in the following 15 base pair sequence): ATATGGTTCTTACCT and the location is 215 base pairs from the 5′ end of the forward primer described in Primer set 2. The sequence of the amplicon should be determined by validated, standard sequencing technologies.

In Invertase Sumner Unit Activity/Analysis/paragraph 4: Change
To prepare the glucose standards, add 1 mL of the Standard sucrose solution to each of the 3 appropriate Sucrose substrate solution test tubes.
to:
To prepare the glucose standards, add 1 mL of the Standard glucose solution to each of the 3 appropriate Sucrose substrate solution test tubes.

In Analysis: Change
Transfer the solution into a glass cylinder with a ground glass stopper and add an equal volume of concentrated hydrochloric acid and 0.25 mL of 5% potassium ferricyanate solution.
to:
Transfer the solution into a glass cylinder with a ground glass stopper and add an equal volume of concentrated hydrochloric acid and 0.25 mL of 5% potassium ferricyanide solution.

In System suitability/Suitability requirements/Resolution: Change
NLT 3.6 between calcium formyltetrahydrofolate (related compound A) and folic acid, System suitability solution
to:
NLT 3.6 between leucovorin calcium and folic acid, System suitability solution

In Phytase Activity: Change
Sample preparation, Procedure 1: Suspend or dissolve and dilute accurately weighed amounts of sample in Acetate buffer so that 2.0 mL of the final dilution (g/mL) will contain between 0.02 and 0.08 phytase units 0.01 and 0.04 FTU/mL.
Sample preparation, Procedure 1: Suspend or dissolve and dilute accurately weighed amounts of sample in Acetate buffer so that the final dilution (g/mL) will contain between 0.01 and 0.04 FTU/mL.
to:
Sample preparation, Procedure 1: Suspend or dissolve and dilute accurately weighed amounts of sample in Acetate buffer so that the final dilution (g/mL) will contain between 0.01 and 0.04 FTU/mL.

In the Acceptance criteria: Change
The spectrum of the sample exhibits absorption bands at approximately the following wavelengths, which correspond in intensity and wavelength to those in the spectrum of the Reference standard (s = strong, m = medium): 1415cm^-1 (s), 1393 cm^-1 (s), 1333 cm^-1 (s), 932 cm^-1 (s), 892 cm^-1 (s), 625 cm^-1 (s), and 604 cm^-1 (m).
to:
The spectrum of the sample exhibits absorption bands at approximately the following wavenumbers, which correspond in intensity and wavenumber to those in the spectrum of the Reference standard (s = strong, m = medium): 1625 cm^-1 (s), 1415 cm^-1 (s), 1393 cm^-1 (s), 1333 cm^-1 (s), 932 cm^-1 (s), 892 cm^-1 (s), and 604 cm^-1 (m).

In Analysis: Change
Each mL of 0.05 M disodium EDTA is equivalent to 8.300 mg of Ca₃(C₆H₅O₇)₂.
to:
Each mL of 0.05 M disodium EDTA is equivalent to 8.307 mg of Ca₃(C₆H₅O₇)₂.