FCC Errata Table - All

In Phytase Activity: Change
Sample preparation, Procedure 1: Suspend or dissolve and dilute accurately weighed amounts of sample in Acetate buffer so that 2.0 mL of the final dilution (g/mL) will contain between 0.02 and 0.08 phytase units 0.01 and 0.04 FTU/mL.
Sample preparation, Procedure 1: Suspend or dissolve and dilute accurately weighed amounts of sample in Acetate buffer so that the final dilution (g/mL) will contain between 0.01 and 0.04 FTU/mL.
to:
Sample preparation, Procedure 1: Suspend or dissolve and dilute accurately weighed amounts of sample in Acetate buffer so that the final dilution (g/mL) will contain between 0.01 and 0.04 FTU/mL.

In System suitability/Suitability requirements/Resolution: Change
NLT 3.6 between calcium formyltetrahydrofolate (related compound A) and folic acid, System suitability solution
to:
NLT 3.6 between leucovorin calcium and folic acid, System suitability solution

In Analysis: Change
Transfer the solution into a glass cylinder with a ground glass stopper and add an equal volume of concentrated hydrochloric acid and 0.25 mL of 5% potassium ferricyanate solution.
to:
Transfer the solution into a glass cylinder with a ground glass stopper and add an equal volume of concentrated hydrochloric acid and 0.25 mL of 5% potassium ferricyanide solution.

In Invertase Sumner Unit Activity/Analysis/paragraph 4: Change
To prepare the glucose standards, add 1 mL of the Standard sucrose solution to each of the 3 appropriate Sucrose substrate solution test tubes.
to:
To prepare the glucose standards, add 1 mL of the Standard glucose solution to each of the 3 appropriate Sucrose substrate solution test tubes.

In the Myristic acid Row and Lower Limit Column of Table 1: Change
4.5
to:
3.0
AND
In the Palmitic acid:Myristic acid ratio Row and Upper Limit Column of Table 1: Change
2.8
to:
3.6
AND
In the Eicosapentaenoic acid Row and Upper Limit Column of Table 1: Change
17.0
to:
19.6

In footnote 12: Change http://www.cast-science.org/publications/index.cfm/probiotics_their_pot….
to:
http://www.cast-science.org.

Line 1 of the second Equation of Analysis: Change
Result = (H_S/H_L) × (RRF_S/W_S) × [(W_L × W_ISS2)/200] × 10⁴
to:
Result = (H_S/H_L) × (RRF_S/W_S) × [(W_L × W_ISS2)/200] × 100

Change the test name
Infrared Absorption
to:
Infrared Spectra
AND
Line 1 of Acceptance criteria: Change
The spectrum of the sample exhibits maxima at the same wavelengths as those in the spectrum displayed below.
to:
The spectrum of the sample exhibits relative maxima at the same wavelengths as those in the spectrum displayed below.

Line 1 of Suitability requirement 2: Change
When injected six consecutive times, the relative standard deviation for the retention time of the advantame peak is NMT 1.0% for the Standard solution having the concentration of USP Advantame RS closest to 160 µg/mL.
to:
When injected six consecutive times, the relative standard deviation of the advantame content (when calculated according to the instructions in the Analysis section) is NMT 3.0% for the Standard solution having the concentration of USP Advantame RS closest to 160 µg/mL.

Line 1 of Primer set 1: Change
The PCR sample preparation prepared with Primer set 1 gives an amplification product of 365 base pairs with two SNPs. The first SNP is identified as (underlined in the following 15 base pair sequence): GAGTAAATTCCATCA and the location is 49 base pairs from the 5′ end of the forward primer described in Primer set 1. The second SNP is identified as (underlined in the following 15 base pair sequence): TTCAATTGAAGTTTG and the location is 241 base pairs from the 5′ end of the forward primer described in Primer set 1. The sequence of the amplicon should be determined by validated, standard sequencing technologies.
to:
The PCR sample preparation prepared with Primer set 1 gives an amplification product of 365 base pairs with two SNPs. The first SNP is identified as (underlined in the following 15 base pair sequence): GAGTAAATTCCATCA and the location is 49 base pairs from the 5′ end of the forward primer described in Primer set 1. The second SNP is identified as (underlined in the following 15 base pair sequence): TTCAATTGAAGTTTG and the location is 241 base pairs from the 5′ end of the forward primer described in Primer set 1. The sequence of the amplicon should be determined by validated, standard sequencing technologies.
AND
Line 1 of Primer set 2: The PCR sample preparation prepared with Primer set 1 gives an amplification product of 433 base pairs with two SNPs. The first SNP is identified as (underlined in the following 15 base pair sequence): AAGCATTCAATACAC and the location is 72 base pairs from the 5′ end of the forward primer described in Primer set 2. The second SNP is identified as (underlined in the following 15 base pair sequence): ATATGGTTCTTACCT and the location is 215 base pairs from the 5′ end of the forward primer described in Primer set 2. The sequence of the amplicon should be determined by validated, standard sequencing technologies.
to:
The PCR sample preparation prepared with Primer set 1 gives an amplification product of 433 base pairs with two SNPs. The first SNP is identified as (underlined in the following 15 base pair sequence): AAGCATTCAATACAC and the location is 72 base pairs from the 5′ end of the forward primer described in Primer set 2. The second SNP is identified as (underlined in the following 15 base pair sequence): ATATGGTTCTTACCT and the location is 215 base pairs from the 5′ end of the forward primer described in Primer set 2. The sequence of the amplicon should be determined by validated, standard sequencing technologies.

Line 1 of Primer set 1: Change
The PCR sample preparation prepared with Primer set 1 gives an amplification product of 351 base pairs with an SNP identified as (underlined in the following 15 base pair sequence): CTTCAGATTTTAGGC. The SNP location is 44 base pairs from the 5′ end of the forward primer described in Primer set 1. The sequence of the amplicon should be determined by validated, standard sequencing technologies.
to:
The PCR sample preparation prepared with Primer set 1 gives an amplification product of 351 base pairs with an SNP identified as (underlined in the following 15 base pair sequence): CTTCAGATTTTAGGC. The SNP location is 44 base pairs from the 5′ end of the forward primer described in Primer set 1. The sequence of the amplicon should be determined by validated, standard sequencing technologies.
AND
Line 1 of Primer set 2: Change
The PCR sample preparation prepared with Primer set 2 gives an amplification product of 531 base pairs with an SNP identified as (underlined in the following 15 base pair sequence): GCCCGCTCAAACGAA. The SNP location is 279 base pairs from the 5′ end of the forward primer described in Primer set 2. The sequence of the amplicon should be determined by validated, standard sequencing technologies.
to:
The PCR sample preparation prepared with Primer set 2 gives an amplification product of 531 base pairs with an SNP identified as (underlined in the following 15 base pair sequence): GCCCGCTCAAACGAA. The SNP location is 279 base pairs from the 5′ end of the forward primer described in Primer set 2. The sequence of the amplicon should be determined by validated, standard sequencing technologies.